Hey, wasn't sure if this should go here or in hardware

the same noise as hardware, but no apparent probs with hardware. Please don't hit me.
Anyways, I was happily playing the Sims 2 (yup, I'm cool) when the computer kept slowing down. It keeps making the same noise as when I connect/disconnect hardware (iPod etc). Only there is definitely no extra stuff plugged in, so no loose connections (on the outside anyway). I checked all the cables at the back, all plugged in securely.
This may seem daft, but can overheating do this? The PC has been on for well over 24 hours.
Any ideas would be greatly appreciate. This is doing my head in!
xxx