HI welcome to PCHF

this could be a number of things can you check somthing for me is sounds like the PC see's the pen drive but occasionally windows will assign it a drive letter that is already taken. Can you try this to change the drive letter if it appears in the
Disk Management to correct it.
How to change a drive letter
To change an existing drive letter on a drive, on a partition, or on a volume, follow these steps: 1.Log on as Administrator or as a member of the Administrators group.2.Click
Start, click
Control Panel. 3.Click
Administrative Tools, double-click
Computer Management, and then click
Disk Management in the left pane.4.Right-click the drive, the partition, the logical drive, or the volume that you want to assign a drive letter to, and then click
Change Drive Letter and Paths.5.Click
Change.6.Click
Assign the following drive letter if it is not already selected, click the drive letter that you want to use, and then click
OK. 7.Click
Yes when you are prompted to confirm the drive letter change.